In vitro antifungal susceptibility of nondermatophytic keratinophilic
Josep Guarro1, Isabel Pujol2, Carmen Aguilar1
and Montserrat Ortoneda1
1Unitat de Microbiologia, Facultat de Medicina, Universitat
Rovira i Virgili and
Nondermatophytic keratinophilic fungi like Scytalidium spp. and Chrysosporium
spp. have been associated with superficial skin infections in humans. However,
there have also been reports of more severe infections both localized and
disseminated caused by these fungi. The best way to treat such infections
has not yet been defined. We have evaluated the in vitro activity of amphotericin
B, flucytosine, fluconazole, ketoconazole, miconazole and itraconazole
against 29 strains of representative species of these two genera (17 strains
of Scytalidium spp. and 12 strains of Chrysosporium spp.), by adapting
the method of the National Committee for Clinical Laboratory Standards
for testing filamentous fungi (M38-P). Amphotericin B and miconazole showed
a very good activity against both genera (all isolates were susceptible
to both drugs). Ketoconazole, fluconazole and itraconazole showed a better
activity against Chrysosporium (all strains were susceptible to ketoconazole
and fluconazole and 25% resistant to itraconazole) than against Scytalidium.
7.69% of the strains of Scytalidium were resistant to ketoconazole, 15.38%
were resistant to fluconazole and 62.50% to itraconazole. Flucytosine was
more active against Scytalidium (23% of the strains resistant) than
against Chrysosporium (all strains resistant). From in vitro data and the
results of some clinical treatments, we conclude that amphotericin B should
be the drug used in the treatment of severe infections by Scytalidium and
2Laboratori de Microbiologia, Hospital Universitari de Sant
Joan de Reus, Reus, Tarragona, Spain
In: Kushwaha RKS, Guarro J (Eds.). Biology
of Dermatophytes and other Keratinophilic Fungi.
2000-2021@ Revista Iberoamericana de Micología. All rights reserved.
Revista Iberoamericana de Micología, Bilbao, 2000.